PNAS/Optimal molecular crowding accelerates group II intron folding and maximizes catalysis [Biochemistry]

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#16186 [ Bishnu P. Paudel, Erica Fiorin ] hit: 7(#f8fff8)


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  DOI: 10.1073/pnas.1806685115
, Unlike in vivo conditions, group II intron ribozymes are known to require high magnesium(II) concentrations ([Mg2+]) and high temperatures (42 °C) for folding and catalysis in vitro. A possible explanation for this difference is the highly crowded cellular environment, which can be mimicked in vitro by macromolecular crowding agents. Here,...