PNAS/Optimal molecular crowding accelerates group II intron folding and maximizes catalysis [Biochemistry]
#16186 [ Bishnu P. Paudel, Erica Fiorin ] 2018-11-20 09:01:25 hit: 7(#f8fff8)
LINK INFOa href="http://www.pnas.org/content/115/47/11917.short?rss=1"
DOI: 10.1073/pnas.1806685115, Unlike in vivo conditions, group II intron ribozymes are known to require high magnesium(II) concentrations ([Mg2+]) and high temperatures (42 °C) for folding and catalysis in vitro. A possible explanation for this difference is the highly crowded cellular environment, which can be mimicked in vitro by macromolecular crowding agents. Here,...