PNAS/Catalytic iron-carbene intermediate revealed in a cytochrome c carbene transferase [Biochemistry]
#16093 [ Russell D. Lewis, Marc Garcia& ] 2018-07-10 09:36:01 hit: 2(#fdfffd)
LINK INFOa href="http://www.pnas.org/content/115/28/7308.short?rss=1"
DOI: 10.1073/pnas.1807027115, Recently, heme proteins have been discovered and engineered by directed evolution to catalyze chemical transformations that are biochemically unprecedented. Many of these nonnatural enzyme-catalyzed reactions are assumed to proceed through a catalytic iron porphyrin carbene (IPC) intermediate, although this intermediate has never been observed in a protein. Using crystallographic, spectroscopic,...